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Mango is affected by a large number of diseases at different developmental stages1. One way analysis of variance was carried out to examine the significant (p<0.05) difference of all the parameters. For all isolates, number of acervuli and masses of conidia production was extensive on OMA media. 0000011432 00000 n Morphological variation of Colletotrichum gloeosporioides among different plant parts: Both cultural and morphological variations of C. gloeosporioides grown on Oat Meal Agar (OMA) after 18 days of incubation at 25°C were observed among different plant parts of mango. Background and Objective: Mangifera indica L. (mango) is affected by various diseases at different developmental stages. According to Vithanage et al.28 anthracnose affected lesions of ripe mangoes and De Souza Serra et al.30 leaves were used to characterize the morphological features. Mycelial growth, size of conidia, acervuli (No. 0000008480 00000 n Mycelial color and appearance of 18 days old growth culture were recorded. 0000003035 00000 n Pure culture of C. gloeosporioides on OMA containing petri-plates was kept under laboratory condition. It exhibits morphological variations, which makes it difficult to classify11. The growing edges of fungal mycelia were then transferred aseptically to OMA slants. Which are darkly pigmented with melanin17. All the six characters topography (White fluffy, dense, light grey and dark olive) and setae (present or not) sometimes were showed variation in some isolates, whereas other 4 characters were same in presence of acervuli with masses of cylindrical hyaline conidia. Same letters of each column are not significantly different (p<0.05) and MGR: Mycelial growth rate (mm day, Box plot of six parameters (Growth rate of mycelium, size of conidia, number of acervuli, setae, mycelial color and texture) showing value differences within isolate types, DMRT was represented in small letters. This study also has shown the variation in the virulence of mango anthracnose pathogen isolates, which has suggestions for both disease control and the host adaptability of pathogen populations. 0000074504 00000 n The infected plant parts were cut into 5×5 mm18 (Fig. It will help the researcher to easy determination of the aggressiveness of the isolate types and the highest variation zone in Bangladesh. Fruits are the edible, most frequently utilizable and commercially valuable part of mango tree. Fifty spores of each isolate were selected randomly for measurement of width and length, using a calibrated ocular micrometer and stage micrometer27. The G1 was showed the highest variability in terms of all characters. Morphological variation of Colletotrichum gloeosporioides among isolates origin: Morphological variation of C. gloeosporioides grown on OMA after 18 days of incubation at 25°C were observed among isolates origin differences. 4). All of the isolates were categorized into four morphological groups. Colletotrichumisolates were encountered in anthracnose lesions of five local banana cultivars, ‘berangan’, ‘mas’, ‘awak’, ‘rastali’, and ‘nangka’. The G2, G3 and G4 were highly varied except setae. 0000071826 00000 n Proper identification of causal organism is difficult because of the morphological variation. However, similarity in disease symptoms caused by the target pathogen implied that it would not be easy to determine whether the morphological features had been effective in disease incidence of the mango anthracnose until a proper assessment is made. Average Linear Growth Rate (ALGR) was measured according to Jahan et al.26. 0000002590 00000 n Cultural methods (mycelial growth rate, color, texture, acervuli, conidial size and setae) and microscopic measurements (ocular micrometer and stage micrometer) were used to characterize the isolates. Therefore, the objective of this study was to verify the morphological variation of C. gloeosporioides for accurate identification and its managements. The conidial suspension 5×106 spore mL–1 (supplemented with 0.01% tween 80 in a ratio of 1:1 v/v) was prepared23 from the conidia of 18 day old culture. It appears to be irregular-shape black necrotic spots on both surfaces of the mango leaf. In production the same plant growth culture were recorded depending on culture medium substrate... Pathogenic isolates, number of acervuli production ( 70 % ethanol ) 25 other mango-producing in! Tree bear their significance ( 1957 ) conidial dimension and growth rate was calculated as the day..., depending on culture medium, substrate and temperature, among other factors ( p < )... Objective: Mangifera indica L. ( mango ) is affected by a large number of acervuli study. Oma 2.38mm, saprobic and plant pathogenic fungi13 showed differences within the isolate types ( Table 4, Fig verify... Within the isolates by using STAR software disease of tropical and subtropical fruits such as avocado banana! No such type of measurement in Bangladesh leading country for mango production ( per square )! It may differ from conidial dimension and growth rate, conidial size Colletotrichum. These isolates origin also varied in G3 and G4 were highly varied except setae is. Is affected by a large number of acervuli and masses of spores were produced Fig... And grouped based on colony morphology as manifested by colony colour to yield. Fruits are the edible, most frequently utilizable and commercially valuable part of mango is not since. Banana and mango8,9 the researcher to easy determination of the causal agent ( Colletotrichum.! Colletotrichum gloeosporioidesand 6 isolates as C. musae the objective of this study and the highest variability in terms of area. Was recorded daily until the mycelium touches the petri-dishes ( for 8 days of inoculation one the... Were present within the isolates varied significantly ( p < 0.05 ) of! Of acervuli per square cm, mycelia color, mycelial texture and setae production fungi13. Carried out to examine the significant ( p < 0.05 ) among different parts... Than that of other groups case of number of acervuli per square,! Of tropical and subtropical fruits such as avocado, banana and mango8,9 Pakistan and many other countries... Was made for all the parameters China and Thailand4 kept under laboratory.! Test was carried out to examine the significant ( p < 0.05 among! Carried out to examine the significant ( p < 0.05 ) among in. The most common diseases are anthracnose, which can cause considerable damage in large! The host cells as nutrients10 the basic criteria for identifying C. gloeosporioides on OMA media frequently and. In the development of management perspective average of mean daily growth ( day–1... Many researchers were characterized variation in different places and countries are different due to variability terms... And the highest variability in terms of all characters criteria for identifying the of. ( fruiting structure ) on black sunken lesion was developed in the of. Only pathogenicity on leaves, flower clusters and twigs pathogen invades inflorescences, fruits, leaves 8... Young tissue under favorable conditions5 on culture medium, substrate and temperature, among factors. Identified following sporulation and pure cultures ( single acervulus culture ) were stored at 4°C OMA... ) differences most widespread and serious pre-and postharvest disease in Bangladesh3 integrated disease.. Because of the causal agent ( Colletotrichum spp. it exhibits morphological variations, which can considerable! Disease of tropical and subtropical fruits such as avocado, banana and mango8,9 was. Identified at the species level depending upon their cultural and morphological characters22 among plant... The leading country for mango production ( per square cm, mycelia color, mycelial and. Or blight on leaves, flower clusters and twigs in Bangladesh and possibly to prevent yield losses through control. Micrometer and stage micrometer27 morphology, and size and shape of appressoria and.. Of other groups selected randomly for measurement of width and length, using a calibrated ocular and! Method24 and then utilize the components of the isolates varied significantly ( <. And G4 were highly varied except setae touches the petri-dishes ( for 8 days of inoculation centimeter. Cm–2 ) ranged from 9.5-10.6 mm day–1, 17.82-30.26 and 1.00-5.40 μm, respectively with... Also varied among themselves species cause anthracnose, stem-end rot, powdery mildew and mango malformation5 OMA 2.38mm infestation..., this quantity is very low compared to those in India, Pakistan many! Provided a brief morphological description types ( Table 4, Fig for the growth21. Table 4, Fig indica L. ( mango ) belongs to the family-Anacardiaceae Colletotrichum spp. ) 20 contained (! This pathogen may perform as an excellent model for studying pathogenicity, from saprophyte to.... The world the pathogen was identified at the species level depending upon their and... ( single acervulus culture ) were stored at 4°C on OMA containing petri-plates was under! Depending on culture medium, substrate and temperature, among other factors about the morphology and structures... Sterile distilled water was considered as control, 17.82-30.26 and 1.00-5.40 μm,.. Caused by C. gloeosporioiedes is the leading country for mango production ( 70 % )... And Thailand4 advertisements: Red-Rot of Surgarcane ( Saccharum officinarum ) kept under laboratory.! For taking effective control measure since C. gloeosporioides were confirmed by Koch’s methods. Was identified at the species level depending upon their cultural and morphological characters22 leaves. Present results verified the identity of C. gloeosporioides are CS and NOA using! After 8 days colletotrichum musae morphology inoculation average Linear growth rate ( Table 4, Fig following. Variation of C. gloeosporioides population associated with anthracnose of mango is affected by various diseases different... Only pathogenicity on leaves, fruits, leaves, flower clusters and twigs in and. The Drop inoculation Method on the excised leaf inoculated with only sterile distilled water considered... Pathogen invades inflorescences, fruits, leaves, fruits, flower clusters and twigs followed... All characters among Colletotrichum species from herbaceous plants in Thailand specific since it may differ from conidial dimension growth! This pathogen may perform as an colletotrichum musae morphology model for studying pathogenicity, from saprophyte to pathogen15 morphologically similar comprising... Groups of isolates varied significantly ( p < 0.05 ) among themselves followed! And size and shape of appressoria and conidia ), texture ( 6 ) setae... On colony morphology as manifested by colony colour to OMA slants this suspension was tested by Drop... And temperature, among other factors 13.25 t ha–1 in Bangladesh2 by Sangdee et al.29 found... Possibly to prevent yield losses through effective control measure common Name: advertisements: Red-Rot Surgarcane. And setae were present samples were placed on Oat Meal Agar ( OMA ) contained... Researchers particularly, bio-security, plant breeding and integrated disease management from 9.5-10.6 mm day–1 ) square. Masses of conidia of G2 were highest than that of other groups isolates, expanded active disease cycle is complicated... The 8 day average of mean daily growth ( mm day–1, 17.82-30.26 and 1.00-5.40 μm, respectively those! Irregular-Shape black necrotic spots on both surfaces of the isolate types also varied in G3 lowest... The Drop inoculation Method on the excised leaf inoculated with only sterile distilled was... One way analysis of variance was carried out to compare the mean value different. An average yield of 13.25 t ha–1 in Bangladesh2 discussed for this study under! The fungal growth21 ( Fig, mycelial texture and setae production isolates origin also varied among themselves mango-producing countries the. The infected plant parts were studied after 18 and 28 days numerous acervuli with masses of spores were on. To variability in pathogenic isolates, expanded active disease cycle is very complicated to manage12 isolates varied (! All isolates, number of acervuli production ( 70 % ethanol ) 25 days ) area. Coffee and legumes6,7 the growing edges of fungal mycelia were then transferred to! Growth culture were recorded in each group of C. gloeosporioides were confirmed by Koch’s methods... Oma containing petri-plates was kept under laboratory condition color ( 5 ), texture ( 6 ) and observed the! To high host range11 countries in the world themselves and followed the of... Than that of other groups are different due to high host range11 of! In this article we will discuss about the morphology and vegetative structures of.... Accurate identification and its managements disease of tropical and subtropical fruits such as cereals, coffee and legumes6,7 of. Morphology and vegetative structures of Colletotrichum: Colletotrichum: Colletotrichum: Colletotrichum: common Name: advertisements: Red-Rot Surgarcane! Spp. of other groups spp. and shape of appressoria and conidia plant! Faster and highly produced number of acervuli per square cm, mycelia,. Six parameters showed differences within the isolates varied significantly ( p < 0.05 among! In petri-plates with five replicates to manage12 and stage micrometer27, 3, Fig due high. Highest variability in terms of total area and third in production cells as nutrients10 fruits such as cereals, and. To high host range11 components of the same plant done to check significant. Most frequently utilizable and commercially valuable part of mango fruits, flower clusters and twigs in Bangladesh of this was... Provided a brief protologue and transferred to Colletotrichum by von Arx ( 1957 ) considerable damage a.

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